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Publication Developmental deletion of amyloid precursor protein precludes transcriptional and proteomic responses to brain injury(Wiley, 2025-04-21) Lacovich, Valentina; Čarna, Maria; Novotný, Sebastian; Wang, Shanshan; Texlová, Kateřina; Kovačovicova, Kristina Locker; Dragišić, Neda; Havas, Daniel; Head, Brian; Geda, Yonas; Limbäck-Stokin, Clara; Stokin, Gorazd Bernard; Stokin, Gorazd Bernard; Medical and DentalIntroduction: Amyloid precursor protein (APP) undergoes striking changes following traumatic brain injury (TBI). Considering its role in the control of gene expression, we investigated whether APP regulates transcription and translation following TBI. Methods: We assessed brain morphology (n = 4-9 mice/group), transcriptome (n = 3 mice/group), proteome (n = 3 mice/group), and behavior (n = 17-27 mice/group) of wild-type (WT) and APP knock-out (KO) mice either untreated or 10-weeks following TBI. Results: After TBI, WT mice displayed transcriptional programs consistent with late stages of brain repair, hub genes were predicted to impact translation and brain proteome showed subtle changes. APP KO mice largely replicated this transcriptional repertoire, but showed no transcriptional nor translational response to TBI. Discussion: The similarities between WT mice following TBI and APP KO mice suggest that developmental APP deficiency induces a condition reminiscent of late stages of brain repair, hampering the control of gene expression in response to injury. Highlights: 10-weeks after TBI, brains exhibit transcriptional profiles consistent with late stage of brain repair. Developmental APP deficiency maintains brains perpetually in an immature state akin to late stages of brain repair. APP responds to TBI by changes in gene expression at a transcriptional and translational level. APP deficiency precludes molecular brain changes in response to TBI.Publication CSP2023: 254 - Improving pathways for patients with acquired brain injury and spinal cord injury through in-depth case review and facilitated workshop(Elsevier, 2024-06-18) Sabapathy, Srikesavan; Jago, Nicky; Gray, Debbie; Sabapathy, Srikesavan; Jago, Nicky; Gray, Debbie; Allied Health Professional; Admin and ClericalNo abstract available